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chiral hplc instrument  (LABOMATIC Instruments)

 
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    LABOMATIC Instruments chiral hplc instrument
    Chiral Hplc Instrument, supplied by LABOMATIC Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chiral+hplc+instrument/chiral+hplc+instrument/us11998539-3940-9-12
    Average 90 stars, based on 1 article reviews
    chiral hplc instrument - by Bioz Stars, 2026-10
    90/100 stars

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    High Performance Liquid Chromatography:

    Article Title: Substituted aminoquinolones as DGKalpha inhibitors for immune activation
    Article Snippet: 85 mg (rac)-6-chloro-1-methyl-4-[4-(5-methyl-1,3-benzoxazol-2-yl)piperidin-1-yl]-2-oxo-7-[(oxolan-3-yl)methoxy]-1,2-dihydroquinoline-3-carboxamide (Example 573) were separated by chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SB 10μ, 250×50; eluent A: methyl tert-butyl ether+0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 60% A+40% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm; 35 mg of the title compound were obtained. .. 85 mg (rac)-6-chloro-1-methyl-4-[4-(5-methyl-1,3-benzoxazol-2-yl)piperidin-1-yl]-2-oxo-7-[(oxolan-3-yl)methoxy]-1,2-dihydroquinoline-3-carboxamide (Example 573) were separated by chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Cellulose SB 10μ, 250×50; eluent A: methyl tert-butyl ether+0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 60% A+40% B; flow: 100 mL/min; temperature: 25° C.; UV: 254 nm; 35 mg of the title compound were obtained. .. Analytical chiral HPLC: R=5.05 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100×4.6; eluent A: methyl tert-butyl ether+0.1 vol % diethylamine; eluent B: methanol; isocratic: 30% A+70% B; flow: 1.4 ml/min; temperature: 25° C.; UV: 254 nm.

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile + 0.1 vol % diethylamine; isocratic: 50% A + 50% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.25 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25°C. .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile + 0.1 vol % diethylamine; isocratic: 50% A + 50% B; flow: 40 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.25 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 50% A + 50% B; flow: 1.4 mL/min; temperature: 25°C. ..

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: Labomatic HD3000, Knauer Pump 100, Labcol Vario 4000 Plus, Knauer DAD 2600; Column: Cellulose SB 5μ 250x50mm Nr. 034; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic: 60% A + 40% B; flow 100.0 mL/min, UV @ 254 nm Analytical chiral HPLC: Rt = 3.40 min Instrument: Agilent HPLC 1260; Column: Cellulose SB 3μ 100x4,6mm; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic: 60% A + 40% B; flow 1.0 mL/min; temperature: 25° C.; DAD 254 nm 59 Intermediate 60; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.77 (s, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.30 (s, 1 H), 7.49-7.53 (m, 3 H), 7.63 (s, 1 H), 7.73-7.79 (m, 2 H), 7.88-8.31 (m, 2 H). .. Preparative chiral HPLC Instrument: Labomatic HD3000, Knauer Pump 100, Labcol Vario 4000 Plus, Knauer DAD 2600; Column: Cellulose SB 5μ 250x50mm Nr. 034; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic: 60% A + 40% B; flow 100.0 mL/min, UV @ 254 nm Analytical chiral HPLC: Rt = 3.40 min Instrument: Agilent HPLC 1260; Column: Cellulose SB 3μ 100x4,6mm; eluent A: hexane + 0.1 Vol-% diethylamine (99%); eluent B: ethanol + 0.1 Vol-% diethylamine (99%); isocratic: 60% A + 40% B; flow 1.0 mL/min; temperature: 25° C.; DAD 254 nm 59 Intermediate 60; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.20 (d, J = 7.35 Hz, 3 H), 3.77 (s, 3 H), 5.03 (q, J = 7.35 Hz, 1 H), 6.94 (d, J = 8.87 Hz, 2 H), 7.30 (s, 1 H), 7.49-7.53 (m, 3 H), 7.63 (s, 1 H), 7.73-7.79 (m, 2 H), 7.88-8.31 (m, 2 H). .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC-4; Column: YMC Amylose SA 5μ, 250 × 30; eluent A: nethyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 70% A + 30% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 1.49 min Instrument: Waters Alliance 2695; Column: YMC Amylose SA 3μ, 100 × 4.6; eluent A: nethyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 70%A + 30% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 59.1 Example 59.1 was determined to be (R)-2-(N-[4- amino-5-(4-methoxybenzoyl)thiazol-2-yl]-4- chloro-3-fluoro-anilino)propanamide, by means of X-ray crystal structure analysis.

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.54 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 49.2 Example 49.2 was determined to be (R)-2-(N-[4- amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2- yl]-3,4-difluoro-anilino)propanamide, by means of X-ray crystal structure analysis. .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.54 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 49.2 Example 49.2 was determined to be (R)-2-(N-[4- amino-5-[4-(difluoromethoxy)benzoyl]thiazol-2- yl]-3,4-difluoro-anilino)propanamide, by means of X-ray crystal structure analysis. ..

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.44 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 44 Intermediate 47; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.10 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.17 (d, J = 8.62 Hz, 2 H), 7.25 (br s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (d, J = 8.87 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 8.21 (brs, 2 H). .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 50 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 5.44 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 44 Intermediate 47; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.10 Hz, 1 H), 7.28 (t, J = 73.76 Hz, 1 H), 7.17 (d, J = 8.62 Hz, 2 H), 7.25 (br s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (d, J = 8.87 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 8.21 (brs, 2 H). ..

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60%A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.69 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 64 Intermediate 87; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (br q, J = 6.84 Hz, 1 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (dd, J = 8.74, 5.45 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.86- 8.56 (m, 2 H). .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 10μ, 250 × 50; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol + 0.1 vol % diethylamine; isocratic: 60%A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.69 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: 2-propanol; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 64 Intermediate 87; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (br q, J = 6.84 Hz, 1 H), 7.22 (t, J = 8.87 Hz, 2 H), 7.25 (s, 1 H), 7.33 (t, J = 8.87 Hz, 2 H), 7.55 (dd, J = 8.74, 5.45 Hz, 2 H), 7.58 (br s, 1 H), 7.64 (dd, J = 8.87, 5.07 Hz, 2 H), 7.86- 8.56 (m, 2 H). ..

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5u, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.99 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 27.2 Example 27 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.60 Hz, 3 H), 5.05 (br d, J =7.10 Hz, 1 H), 7.19-7.25 (m, 2 H), 7.28 (s, 1 H), 7.47-7.53 (m, 2 H), 7.53- 7.59 (m, 2 H), 7.59-7.63 (m, 1 H), 7.70-7.76 (m, 2 H), 7.96- 8.40 (m, 2 H). .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5u, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 2.99 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100 × 4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 27.2 Example 27 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.17 (d, J = 7.60 Hz, 3 H), 5.05 (br d, J =7.10 Hz, 1 H), 7.19-7.25 (m, 2 H), 7.28 (s, 1 H), 7.47-7.53 (m, 2 H), 7.53- 7.59 (m, 2 H), 7.59-7.63 (m, 1 H), 7.70-7.76 (m, 2 H), 7.96- 8.40 (m, 2 H). .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SB 5μ, 250 × 30; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol + 0.1 vol % diethylamine; isocratic: 80% A + 20% B; flow: 60 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.81 min Instrument: Waters Alliance 2695; Column: YMC Cellulose SB 3μ, 100x4.6; eluent A: hexane + 0.1 vol % diethylamine; eluent B: ethanol; isocratic: 80% A + 20% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 28 Intermediate 31; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.18 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.52 Hz, 1 H), 7.30 (s, 1 H), 7.36-7.44 (m, 3 H), 7.47-7.52 (m, 2 H), 7.63 (s, 1 H), 7.80-7.91 (m, 4 H), 8.18 (br s, 2 H).

    Article Title: Substituted aminothiazoles as DGKzeta inhibitors for immune activation
    Article Snippet: Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.34 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 57 Intermediate 58; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.27 Hz, 1 H), 7.27 (s, 1 H), 7.41 (d, J = 6.08 Hz, 2 H), 7.59 (d, J = 10.90 Hz, 5 H), 8.08-8.48 (m, 2 H), 8.62 (d, J = 6.08 Hz, 2 H). .. Preparative chiral HPLC Instrument: PrepCon Labomatic HPLC; Column: YMC Cellulose SC 10μ, 250 × 50; eluent A: methyl tert-butyl ether; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 140 mL/min; temperature: 25° C.; UV: 254 nm Analytical chiral HPLC: Rt = 3.34 min Instrument: Thermo Fisher UltiMate 3000; Column: YMC Cellulose SC 3μ, 100 × 4.6; eluent A: methyl tert-butyl ether + 0.1 vol % diethylamine; eluent B: acetonitrile; isocratic: 60% A + 40% B; flow: 1.4 mL/min; temperature: 25° C.; UV: 254 nm 57 Intermediate 58; rac-2- bromo- propanamide 1H-NMR (400 MHz, DMSO-d6): δ ppm = 1.16 (d, J = 7.35 Hz, 3 H), 5.06 (q, J = 7.27 Hz, 1 H), 7.27 (s, 1 H), 7.41 (d, J = 6.08 Hz, 2 H), 7.59 (d, J = 10.90 Hz, 5 H), 8.08-8.48 (m, 2 H), 8.62 (d, J = 6.08 Hz, 2 H). .. Preparative chiral HPLC Instrument: Sepiatec: Prep SFC100; Column: Chiralpak IG 5μ 250 × 30mm; eluent A: CO2; eluent B: 6 thanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 100 mL/min; temperature: 40° C.; BPR: 150 bar; UV: 254 nm Analytical chiral HPLC: Rt = 4.76 min Instrument: Agilent: 1260, Aurora SFC-Modul; Column: Chiralpak IG 5μ 100 × 4.6 mm; eluent A: CO2; eluent B: ethanol + 0.2 vol % aqueous ammonia (32%); isocratic: 30% B; flow: 4 mL/min; temperature: 37.5° C.; BPR: 100 bar; UV: 254 nm 57.2 Example 57 1H NMR (400 MHz, DMSO-d6) δ ppm = 1.17 (d, J =7.35 Hz, 3 H), 5.06 (m, 1 H), 7.28 (s, 1 H), 7.41 (m, 2 H), 7.59 (m, 5 H), 8.28 (m, 2 H), 8.62 (m, 2 H) LC-MS (method 1) Rt = 0.85 min MS (ESIpos): m/z = 402.4 [M + H]+ [α]D20 = 76° c = 9.6 mg/mL in DMSO 36% yield 2-(N-[4-amino-5-(pyridine-4- carbonyl)thiazol-2-yl]-4-chloro- anilino)propanamide (enantiomer 2) Chiral HPLC Example 57.2 HPLC separation of rac-2-(N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro- anilino)propanamide (590 mg, 1.47 mmol, Example 57) on a chiral column gave 225 mg (36% yield) of 2 (N-[4-amino-5-(pyridine-4-carbonyl)thiazol-2-yl]-4-chloro-anilino)propanamide, enantiomer 2.



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